ASTM E Antimicrobial Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. The test.
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The w2149 of viable organisms from the suspension is determined and the percent reduction is calculated by comparing retrievals from appropriate controls. This standard does not purport to address all of the safety concerns, if any, associated with its use.
One-hour contact time in a buffer solution allows for metabolic stasis in the population. Record and report presence of solution activity. The ASTM E Standard Test Method is used to evaluate the antimicrobial activity of substrate-bound, non-leaching, antimicrobial-treated samples under dynamic contact conditions.
Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard. Work Item s – proposed revisions of this standard. The flask is placed onto a wrist-action shaker and shaken for a desired exposure time, typically 1 hour.
ASTM E – Microbe Investigations (MIS)
This method is generally used to substantiate antimicrobial properties of treated specimens for non-public health related claims. These difficulties include ensuring contact asstm inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility.
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If an antimicrobial agent that is shown to be removed asttm the surface by Section 13 is utilized in this test methodology, controls must be included such that appropriate neutralization steps are including during recovery and enumeration.
Link to Active This link will always route to the current Active version of the standard.
It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. The antimicrobial activity of a substrate-bound, non-leaching antimicrobial agent is dependent upon direct contact of microbes with the active chemical agent.
Summary of Test In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli.
This standard does not purport to address all of the safety concerns, if any, associated with its use. Active view current version of standard. This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism.
No other units of measurement are included in this standard. These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility. This test method is designed to evaluate the resistance of specimens treated with a non-leaching antimicrobial agent to the growth of microbes under dynamic contact conditions.
The test determines the antimicrobial activity of a treated specimen by shaking samples of surface-bound materials in a concentrated bacterial suspension for a one hour contact time.
Summary of F2149 Method: It is for this reason that the use of the term leaching throughout this document is limited to only the testing conditions described herein. This website stores cookies on your computer.
This test method is only intended to determine efficacy as described in 4. This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials.
The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined for e21149 test flask as compared to the untreated control suspension. Following exposure, a sample of the test organism suspension is removed quantitatively assayed for survivors.
ASTM E2149 Antimicrobial Activity
The susceptibility of the species to particular biocides could be altered depending on its life stage cycle. Materials evaluated by this method may be comprised of fabric, paper, powder or other solid materials. This dynamic shake flash test was developed for routine quality control and screening test. Facebook Linkedin Instagram Twitter Google.